Isolation and identification of non-saccharomyces ethanol and thermo-tolerant yeasts strains from fermented carbohydrate wastes

Ethanol is an important chemical with its potential as a biofuel to displace the environmentally unfriendly fossil fuels. Isolation and identification of ethanol thermo-tolerant yeast capable producing were carried out in this study. Seventy (70) samples from different fermented foods agricultural wastes millets, sorghum, maize, rice guinea corn obtained local sellers Panteka, Farin-Gida, Barkallahu Barnawa Kaduna State (North West), Nigeria used research. The collected using sterile plastic container transported immediately Biotechnology laboratory Nigerian Defence Academy iced chamber. They serially diluted distilled water inoculated on extract peptone dextrose agar (YEPD) media plates supplemented 0.2% chloramphenicol. incubated at 30°C for 72 hours developed colonies sub-cultured obtain pure culture which was observed their morphological microscopic characteristics. After examination, ten (10) isolates seventy collected. Flocculation test, Hydrogen sulphide preliminary alcohol production sugar fermentation test tolerance varying concentration 5%-20% (v/v) temperature 37oC-45oC studied. Using internal transcribed spacer (ITS) 1 2 regions primer, DNA sequenced identified. Four FG 17, 27, P1 BN50 showed potentials 20% identified strains Diutina rugosa, Candida sp, ethanolica tropicalis while one isolate 35 good thermo-tolerance ability 45oC Trichosporon sp.